The Recombinant Protein Expression Services group develops and produces biotherapeutics for pharmaceuticals and clinical diagnostics, and cell lines over-expressing cell surface receptors or other target proteins for development of high-throughput in vitro assay screens.

Avesthagen's combined expertise in cell culture, cell line development, and cell and molecular biology enables us to design and perform novel cell-based assays or to perform existing assays on a contract basis. Avesthagen also offers a broad range of custom-designed protein expression and purification services that complement its expertise in cell culture and Cell Line Development. Our protein expression and purification services are tailored to the specific needs and preferences of our clients. Avesthagen experienced scientists will work with you to design optimized work plans that meet your expectations regarding epitope-tagging of recombinant proteins, expression analysis, expression optimization, production scale, product purity, product activity, etc.

• Development of novel expression protocols for recombinant protein products in bacteria, yeast and mammalian cells
• Development of recombinant mammalian cell lines (CHO, HEK293) over-expressing cell-surface receptors to carry out invitro assays.

Select Projects

• Cloning and expression of a protein in bacteria, yeast for immunodiagnostics reagents
• cloning and expression of a protein in mammalian cells for development of a biotherapeutic for rheumatoid arthritis
• Cloning and expression of several GPCR proteins in mammalian cells for development of cell lines used in HTS

Technologies / Infrastructure

• high-level expression of proteins in bacteria, yeast and mammalian systems
• Protein purification using FPLC and HPLC
• Pilot plant facility for scale-up